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hematoxylin bluing solution  (Servicebio Inc)

 
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    Servicebio Inc hematoxylin bluing solution
    Hematoxylin Bluing Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hematoxylin+bluing+solution/differentiation+hematoxylin+solution/pm41892956-221-29-39
    Average 86 stars, based on 1 article reviews
    hematoxylin bluing solution - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Anti-Photoaging Effect of Pinctada martensii Hydrolysates on Ultraviolet B-Irradiated Nude Mice Skin.
    Article Snippet: .. After deparaffinization, sections were incubated with a high-definition staining pretreatment solution for 1 min, stained with hematoxylin for 3–5 min, differentiated with a hematoxylin differentiation solution, blued with a hematoxylin bluing solution, and counterstained with eosin for 15 s (Servicebio, Wuhan, China). ..

    Article Title: Anti-Photoaging Effect of Pinctada martensii Hydrolysates on Ultraviolet B-Irradiated Nude Mice Skin
    Article Snippet: .. After deparaffinization, sections were incubated with a high-definition staining pretreatment solution for 1 min, stained with hematoxylin for 3–5 min, differentiated with a hematoxylin differentiation solution, blued with a hematoxylin bluing solution, and counterstained with eosin for 15 s (Servicebio, Wuhan, China). ..

    Staining:

    Article Title: Anti-Photoaging Effect of Pinctada martensii Hydrolysates on Ultraviolet B-Irradiated Nude Mice Skin.
    Article Snippet: .. After deparaffinization, sections were incubated with a high-definition staining pretreatment solution for 1 min, stained with hematoxylin for 3–5 min, differentiated with a hematoxylin differentiation solution, blued with a hematoxylin bluing solution, and counterstained with eosin for 15 s (Servicebio, Wuhan, China). ..

    Article Title: Mitigating effects of Jiawei Chaihu Shugan decoction on necroptosis and inflammation of hippocampal neurons in epileptic mice.
    Article Snippet: Sixty 6-wk-old male SPF C57BL/6J mice were purchased from Beijing Sibeifu Biotechnology Co., Ltd (License No. SYXK (Gui) 2009-0001). .. The following items were obtained from the indicated supplier: complete DMEM (KGM12800S, Kaiji Bio), incomplete DMEM (KGM12800N, Kaiji Bio), FBS (10099-141, Gibco), Lipofectamine 3000 transfection reagent (L3000015, Invitrogen), OptiMEM (31985-062, Gibco), trypsin-EDTA digestion solution (T1300, Solarbio), 1× PBS (0.01 M, pH7.4) (KGB5001, Kaiji Bio), Trizon reagent (CW0580S, CWBIO), ultrapure RNA extraction kit (CW0581M, CWBIO), HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) (R223-01, Vazyme), ChamQ Universal SYBR qPCR master mix (Q711-02, Vazyme), RIPA lysis buffer (C1053, Beijing Prumeikai Gene Technology Co., Ltd.), BCA protein quantitation kit (E-BC-K318-M, Elabscience), sodium dodecyl sulfate (SDS) (151-21-3, Xilong Science Co., Ltd.), PVDF membranes (IPVH00010, Millipore), blocking-specific powdered skim milk (P1622, Beijing Prumeikai Gene Technology Co., Ltd.), DAB staining kit (CW0125M, CWBIO), xylene (33535, Xilong Science Co., Ltd.), anhydrous ethanol (32061, Xilong Science Co., Ltd.), 95% ethanol (32061, Xilong Science Co., Ltd.), TUNEL detection kit (red) (C1090, Beyotime), hematoxylin stain (ZLI-9610, Zhongshan Golden Bridge), eosin stain (G1100, Solarbio), hematoxylin bluing solution (G1040, Servicebio), ultra-clean mounting medium (YZB, BASO). .. The following antibodies were obtained from the indicated suppliers: internal control primary antibody, mouse anti-β-actin (HC201, TransGen Biotech); target primary antibodies: rabbit anti-Csnk1g3-85aa synthesized by Hangzhou Huaan Biotechnology Co., Ltd, rabbit anti-CK1γ3 (AF9034, Affinity), rabbit anti-RIP1 (13176-AP, Proteintech), rabbit anti-RIP3 (AF7942, Affinity), rabbit anti-MLKL (OM155258, OmnimAbs), mouse anti-TNF-α (60291-1-lg, Proteintech), rabbit anti-IL-1β (sc-32294, Santa Cruz Biotechnology), rabbit anti-IL-6 (DF6087, Affinity); secondary antibodies: HRP-conjugated goat anti-rabbit IgG (H + L) (GB23303, Servicebio) and HRP-conjugated goat anti-mouse IgG (H + L) (GB23301, Servicebio).

    Article Title: Anti-Photoaging Effect of Pinctada martensii Hydrolysates on Ultraviolet B-Irradiated Nude Mice Skin
    Article Snippet: .. After deparaffinization, sections were incubated with a high-definition staining pretreatment solution for 1 min, stained with hematoxylin for 3–5 min, differentiated with a hematoxylin differentiation solution, blued with a hematoxylin bluing solution, and counterstained with eosin for 15 s (Servicebio, Wuhan, China). ..

    Article Title: Protein restriction reprograms the multi-organ proteomic landscape of mouse aging.
    Article Snippet: Resource Protein restriction reprograms the multi-organ proteomic landscape of mouse aging

    Transfection:

    Article Title: Mitigating effects of Jiawei Chaihu Shugan decoction on necroptosis and inflammation of hippocampal neurons in epileptic mice.
    Article Snippet: Sixty 6-wk-old male SPF C57BL/6J mice were purchased from Beijing Sibeifu Biotechnology Co., Ltd (License No. SYXK (Gui) 2009-0001). .. The following items were obtained from the indicated supplier: complete DMEM (KGM12800S, Kaiji Bio), incomplete DMEM (KGM12800N, Kaiji Bio), FBS (10099-141, Gibco), Lipofectamine 3000 transfection reagent (L3000015, Invitrogen), OptiMEM (31985-062, Gibco), trypsin-EDTA digestion solution (T1300, Solarbio), 1× PBS (0.01 M, pH7.4) (KGB5001, Kaiji Bio), Trizon reagent (CW0580S, CWBIO), ultrapure RNA extraction kit (CW0581M, CWBIO), HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) (R223-01, Vazyme), ChamQ Universal SYBR qPCR master mix (Q711-02, Vazyme), RIPA lysis buffer (C1053, Beijing Prumeikai Gene Technology Co., Ltd.), BCA protein quantitation kit (E-BC-K318-M, Elabscience), sodium dodecyl sulfate (SDS) (151-21-3, Xilong Science Co., Ltd.), PVDF membranes (IPVH00010, Millipore), blocking-specific powdered skim milk (P1622, Beijing Prumeikai Gene Technology Co., Ltd.), DAB staining kit (CW0125M, CWBIO), xylene (33535, Xilong Science Co., Ltd.), anhydrous ethanol (32061, Xilong Science Co., Ltd.), 95% ethanol (32061, Xilong Science Co., Ltd.), TUNEL detection kit (red) (C1090, Beyotime), hematoxylin stain (ZLI-9610, Zhongshan Golden Bridge), eosin stain (G1100, Solarbio), hematoxylin bluing solution (G1040, Servicebio), ultra-clean mounting medium (YZB, BASO). .. The following antibodies were obtained from the indicated suppliers: internal control primary antibody, mouse anti-β-actin (HC201, TransGen Biotech); target primary antibodies: rabbit anti-Csnk1g3-85aa synthesized by Hangzhou Huaan Biotechnology Co., Ltd, rabbit anti-CK1γ3 (AF9034, Affinity), rabbit anti-RIP1 (13176-AP, Proteintech), rabbit anti-RIP3 (AF7942, Affinity), rabbit anti-MLKL (OM155258, OmnimAbs), mouse anti-TNF-α (60291-1-lg, Proteintech), rabbit anti-IL-1β (sc-32294, Santa Cruz Biotechnology), rabbit anti-IL-6 (DF6087, Affinity); secondary antibodies: HRP-conjugated goat anti-rabbit IgG (H + L) (GB23303, Servicebio) and HRP-conjugated goat anti-mouse IgG (H + L) (GB23301, Servicebio).

    RNA Extraction:

    Article Title: Mitigating effects of Jiawei Chaihu Shugan decoction on necroptosis and inflammation of hippocampal neurons in epileptic mice.
    Article Snippet: Sixty 6-wk-old male SPF C57BL/6J mice were purchased from Beijing Sibeifu Biotechnology Co., Ltd (License No. SYXK (Gui) 2009-0001). .. The following items were obtained from the indicated supplier: complete DMEM (KGM12800S, Kaiji Bio), incomplete DMEM (KGM12800N, Kaiji Bio), FBS (10099-141, Gibco), Lipofectamine 3000 transfection reagent (L3000015, Invitrogen), OptiMEM (31985-062, Gibco), trypsin-EDTA digestion solution (T1300, Solarbio), 1× PBS (0.01 M, pH7.4) (KGB5001, Kaiji Bio), Trizon reagent (CW0580S, CWBIO), ultrapure RNA extraction kit (CW0581M, CWBIO), HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) (R223-01, Vazyme), ChamQ Universal SYBR qPCR master mix (Q711-02, Vazyme), RIPA lysis buffer (C1053, Beijing Prumeikai Gene Technology Co., Ltd.), BCA protein quantitation kit (E-BC-K318-M, Elabscience), sodium dodecyl sulfate (SDS) (151-21-3, Xilong Science Co., Ltd.), PVDF membranes (IPVH00010, Millipore), blocking-specific powdered skim milk (P1622, Beijing Prumeikai Gene Technology Co., Ltd.), DAB staining kit (CW0125M, CWBIO), xylene (33535, Xilong Science Co., Ltd.), anhydrous ethanol (32061, Xilong Science Co., Ltd.), 95% ethanol (32061, Xilong Science Co., Ltd.), TUNEL detection kit (red) (C1090, Beyotime), hematoxylin stain (ZLI-9610, Zhongshan Golden Bridge), eosin stain (G1100, Solarbio), hematoxylin bluing solution (G1040, Servicebio), ultra-clean mounting medium (YZB, BASO). .. The following antibodies were obtained from the indicated suppliers: internal control primary antibody, mouse anti-β-actin (HC201, TransGen Biotech); target primary antibodies: rabbit anti-Csnk1g3-85aa synthesized by Hangzhou Huaan Biotechnology Co., Ltd, rabbit anti-CK1γ3 (AF9034, Affinity), rabbit anti-RIP1 (13176-AP, Proteintech), rabbit anti-RIP3 (AF7942, Affinity), rabbit anti-MLKL (OM155258, OmnimAbs), mouse anti-TNF-α (60291-1-lg, Proteintech), rabbit anti-IL-1β (sc-32294, Santa Cruz Biotechnology), rabbit anti-IL-6 (DF6087, Affinity); secondary antibodies: HRP-conjugated goat anti-rabbit IgG (H + L) (GB23303, Servicebio) and HRP-conjugated goat anti-mouse IgG (H + L) (GB23301, Servicebio).

    Real-time Polymerase Chain Reaction:

    Article Title: Mitigating effects of Jiawei Chaihu Shugan decoction on necroptosis and inflammation of hippocampal neurons in epileptic mice.
    Article Snippet: Sixty 6-wk-old male SPF C57BL/6J mice were purchased from Beijing Sibeifu Biotechnology Co., Ltd (License No. SYXK (Gui) 2009-0001). .. The following items were obtained from the indicated supplier: complete DMEM (KGM12800S, Kaiji Bio), incomplete DMEM (KGM12800N, Kaiji Bio), FBS (10099-141, Gibco), Lipofectamine 3000 transfection reagent (L3000015, Invitrogen), OptiMEM (31985-062, Gibco), trypsin-EDTA digestion solution (T1300, Solarbio), 1× PBS (0.01 M, pH7.4) (KGB5001, Kaiji Bio), Trizon reagent (CW0580S, CWBIO), ultrapure RNA extraction kit (CW0581M, CWBIO), HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) (R223-01, Vazyme), ChamQ Universal SYBR qPCR master mix (Q711-02, Vazyme), RIPA lysis buffer (C1053, Beijing Prumeikai Gene Technology Co., Ltd.), BCA protein quantitation kit (E-BC-K318-M, Elabscience), sodium dodecyl sulfate (SDS) (151-21-3, Xilong Science Co., Ltd.), PVDF membranes (IPVH00010, Millipore), blocking-specific powdered skim milk (P1622, Beijing Prumeikai Gene Technology Co., Ltd.), DAB staining kit (CW0125M, CWBIO), xylene (33535, Xilong Science Co., Ltd.), anhydrous ethanol (32061, Xilong Science Co., Ltd.), 95% ethanol (32061, Xilong Science Co., Ltd.), TUNEL detection kit (red) (C1090, Beyotime), hematoxylin stain (ZLI-9610, Zhongshan Golden Bridge), eosin stain (G1100, Solarbio), hematoxylin bluing solution (G1040, Servicebio), ultra-clean mounting medium (YZB, BASO). .. The following antibodies were obtained from the indicated suppliers: internal control primary antibody, mouse anti-β-actin (HC201, TransGen Biotech); target primary antibodies: rabbit anti-Csnk1g3-85aa synthesized by Hangzhou Huaan Biotechnology Co., Ltd, rabbit anti-CK1γ3 (AF9034, Affinity), rabbit anti-RIP1 (13176-AP, Proteintech), rabbit anti-RIP3 (AF7942, Affinity), rabbit anti-MLKL (OM155258, OmnimAbs), mouse anti-TNF-α (60291-1-lg, Proteintech), rabbit anti-IL-1β (sc-32294, Santa Cruz Biotechnology), rabbit anti-IL-6 (DF6087, Affinity); secondary antibodies: HRP-conjugated goat anti-rabbit IgG (H + L) (GB23303, Servicebio) and HRP-conjugated goat anti-mouse IgG (H + L) (GB23301, Servicebio).

    Lysis:

    Article Title: Mitigating effects of Jiawei Chaihu Shugan decoction on necroptosis and inflammation of hippocampal neurons in epileptic mice.
    Article Snippet: Sixty 6-wk-old male SPF C57BL/6J mice were purchased from Beijing Sibeifu Biotechnology Co., Ltd (License No. SYXK (Gui) 2009-0001). .. The following items were obtained from the indicated supplier: complete DMEM (KGM12800S, Kaiji Bio), incomplete DMEM (KGM12800N, Kaiji Bio), FBS (10099-141, Gibco), Lipofectamine 3000 transfection reagent (L3000015, Invitrogen), OptiMEM (31985-062, Gibco), trypsin-EDTA digestion solution (T1300, Solarbio), 1× PBS (0.01 M, pH7.4) (KGB5001, Kaiji Bio), Trizon reagent (CW0580S, CWBIO), ultrapure RNA extraction kit (CW0581M, CWBIO), HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) (R223-01, Vazyme), ChamQ Universal SYBR qPCR master mix (Q711-02, Vazyme), RIPA lysis buffer (C1053, Beijing Prumeikai Gene Technology Co., Ltd.), BCA protein quantitation kit (E-BC-K318-M, Elabscience), sodium dodecyl sulfate (SDS) (151-21-3, Xilong Science Co., Ltd.), PVDF membranes (IPVH00010, Millipore), blocking-specific powdered skim milk (P1622, Beijing Prumeikai Gene Technology Co., Ltd.), DAB staining kit (CW0125M, CWBIO), xylene (33535, Xilong Science Co., Ltd.), anhydrous ethanol (32061, Xilong Science Co., Ltd.), 95% ethanol (32061, Xilong Science Co., Ltd.), TUNEL detection kit (red) (C1090, Beyotime), hematoxylin stain (ZLI-9610, Zhongshan Golden Bridge), eosin stain (G1100, Solarbio), hematoxylin bluing solution (G1040, Servicebio), ultra-clean mounting medium (YZB, BASO). .. The following antibodies were obtained from the indicated suppliers: internal control primary antibody, mouse anti-β-actin (HC201, TransGen Biotech); target primary antibodies: rabbit anti-Csnk1g3-85aa synthesized by Hangzhou Huaan Biotechnology Co., Ltd, rabbit anti-CK1γ3 (AF9034, Affinity), rabbit anti-RIP1 (13176-AP, Proteintech), rabbit anti-RIP3 (AF7942, Affinity), rabbit anti-MLKL (OM155258, OmnimAbs), mouse anti-TNF-α (60291-1-lg, Proteintech), rabbit anti-IL-1β (sc-32294, Santa Cruz Biotechnology), rabbit anti-IL-6 (DF6087, Affinity); secondary antibodies: HRP-conjugated goat anti-rabbit IgG (H + L) (GB23303, Servicebio) and HRP-conjugated goat anti-mouse IgG (H + L) (GB23301, Servicebio).

    Protein Quantitation:

    Article Title: Mitigating effects of Jiawei Chaihu Shugan decoction on necroptosis and inflammation of hippocampal neurons in epileptic mice.
    Article Snippet: Sixty 6-wk-old male SPF C57BL/6J mice were purchased from Beijing Sibeifu Biotechnology Co., Ltd (License No. SYXK (Gui) 2009-0001). .. The following items were obtained from the indicated supplier: complete DMEM (KGM12800S, Kaiji Bio), incomplete DMEM (KGM12800N, Kaiji Bio), FBS (10099-141, Gibco), Lipofectamine 3000 transfection reagent (L3000015, Invitrogen), OptiMEM (31985-062, Gibco), trypsin-EDTA digestion solution (T1300, Solarbio), 1× PBS (0.01 M, pH7.4) (KGB5001, Kaiji Bio), Trizon reagent (CW0580S, CWBIO), ultrapure RNA extraction kit (CW0581M, CWBIO), HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) (R223-01, Vazyme), ChamQ Universal SYBR qPCR master mix (Q711-02, Vazyme), RIPA lysis buffer (C1053, Beijing Prumeikai Gene Technology Co., Ltd.), BCA protein quantitation kit (E-BC-K318-M, Elabscience), sodium dodecyl sulfate (SDS) (151-21-3, Xilong Science Co., Ltd.), PVDF membranes (IPVH00010, Millipore), blocking-specific powdered skim milk (P1622, Beijing Prumeikai Gene Technology Co., Ltd.), DAB staining kit (CW0125M, CWBIO), xylene (33535, Xilong Science Co., Ltd.), anhydrous ethanol (32061, Xilong Science Co., Ltd.), 95% ethanol (32061, Xilong Science Co., Ltd.), TUNEL detection kit (red) (C1090, Beyotime), hematoxylin stain (ZLI-9610, Zhongshan Golden Bridge), eosin stain (G1100, Solarbio), hematoxylin bluing solution (G1040, Servicebio), ultra-clean mounting medium (YZB, BASO). .. The following antibodies were obtained from the indicated suppliers: internal control primary antibody, mouse anti-β-actin (HC201, TransGen Biotech); target primary antibodies: rabbit anti-Csnk1g3-85aa synthesized by Hangzhou Huaan Biotechnology Co., Ltd, rabbit anti-CK1γ3 (AF9034, Affinity), rabbit anti-RIP1 (13176-AP, Proteintech), rabbit anti-RIP3 (AF7942, Affinity), rabbit anti-MLKL (OM155258, OmnimAbs), mouse anti-TNF-α (60291-1-lg, Proteintech), rabbit anti-IL-1β (sc-32294, Santa Cruz Biotechnology), rabbit anti-IL-6 (DF6087, Affinity); secondary antibodies: HRP-conjugated goat anti-rabbit IgG (H + L) (GB23303, Servicebio) and HRP-conjugated goat anti-mouse IgG (H + L) (GB23301, Servicebio).

    Blocking Assay:

    Article Title: Mitigating effects of Jiawei Chaihu Shugan decoction on necroptosis and inflammation of hippocampal neurons in epileptic mice.
    Article Snippet: Sixty 6-wk-old male SPF C57BL/6J mice were purchased from Beijing Sibeifu Biotechnology Co., Ltd (License No. SYXK (Gui) 2009-0001). .. The following items were obtained from the indicated supplier: complete DMEM (KGM12800S, Kaiji Bio), incomplete DMEM (KGM12800N, Kaiji Bio), FBS (10099-141, Gibco), Lipofectamine 3000 transfection reagent (L3000015, Invitrogen), OptiMEM (31985-062, Gibco), trypsin-EDTA digestion solution (T1300, Solarbio), 1× PBS (0.01 M, pH7.4) (KGB5001, Kaiji Bio), Trizon reagent (CW0580S, CWBIO), ultrapure RNA extraction kit (CW0581M, CWBIO), HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) (R223-01, Vazyme), ChamQ Universal SYBR qPCR master mix (Q711-02, Vazyme), RIPA lysis buffer (C1053, Beijing Prumeikai Gene Technology Co., Ltd.), BCA protein quantitation kit (E-BC-K318-M, Elabscience), sodium dodecyl sulfate (SDS) (151-21-3, Xilong Science Co., Ltd.), PVDF membranes (IPVH00010, Millipore), blocking-specific powdered skim milk (P1622, Beijing Prumeikai Gene Technology Co., Ltd.), DAB staining kit (CW0125M, CWBIO), xylene (33535, Xilong Science Co., Ltd.), anhydrous ethanol (32061, Xilong Science Co., Ltd.), 95% ethanol (32061, Xilong Science Co., Ltd.), TUNEL detection kit (red) (C1090, Beyotime), hematoxylin stain (ZLI-9610, Zhongshan Golden Bridge), eosin stain (G1100, Solarbio), hematoxylin bluing solution (G1040, Servicebio), ultra-clean mounting medium (YZB, BASO). .. The following antibodies were obtained from the indicated suppliers: internal control primary antibody, mouse anti-β-actin (HC201, TransGen Biotech); target primary antibodies: rabbit anti-Csnk1g3-85aa synthesized by Hangzhou Huaan Biotechnology Co., Ltd, rabbit anti-CK1γ3 (AF9034, Affinity), rabbit anti-RIP1 (13176-AP, Proteintech), rabbit anti-RIP3 (AF7942, Affinity), rabbit anti-MLKL (OM155258, OmnimAbs), mouse anti-TNF-α (60291-1-lg, Proteintech), rabbit anti-IL-1β (sc-32294, Santa Cruz Biotechnology), rabbit anti-IL-6 (DF6087, Affinity); secondary antibodies: HRP-conjugated goat anti-rabbit IgG (H + L) (GB23303, Servicebio) and HRP-conjugated goat anti-mouse IgG (H + L) (GB23301, Servicebio).

    TUNEL Assay:

    Article Title: Mitigating effects of Jiawei Chaihu Shugan decoction on necroptosis and inflammation of hippocampal neurons in epileptic mice.
    Article Snippet: Sixty 6-wk-old male SPF C57BL/6J mice were purchased from Beijing Sibeifu Biotechnology Co., Ltd (License No. SYXK (Gui) 2009-0001). .. The following items were obtained from the indicated supplier: complete DMEM (KGM12800S, Kaiji Bio), incomplete DMEM (KGM12800N, Kaiji Bio), FBS (10099-141, Gibco), Lipofectamine 3000 transfection reagent (L3000015, Invitrogen), OptiMEM (31985-062, Gibco), trypsin-EDTA digestion solution (T1300, Solarbio), 1× PBS (0.01 M, pH7.4) (KGB5001, Kaiji Bio), Trizon reagent (CW0580S, CWBIO), ultrapure RNA extraction kit (CW0581M, CWBIO), HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) (R223-01, Vazyme), ChamQ Universal SYBR qPCR master mix (Q711-02, Vazyme), RIPA lysis buffer (C1053, Beijing Prumeikai Gene Technology Co., Ltd.), BCA protein quantitation kit (E-BC-K318-M, Elabscience), sodium dodecyl sulfate (SDS) (151-21-3, Xilong Science Co., Ltd.), PVDF membranes (IPVH00010, Millipore), blocking-specific powdered skim milk (P1622, Beijing Prumeikai Gene Technology Co., Ltd.), DAB staining kit (CW0125M, CWBIO), xylene (33535, Xilong Science Co., Ltd.), anhydrous ethanol (32061, Xilong Science Co., Ltd.), 95% ethanol (32061, Xilong Science Co., Ltd.), TUNEL detection kit (red) (C1090, Beyotime), hematoxylin stain (ZLI-9610, Zhongshan Golden Bridge), eosin stain (G1100, Solarbio), hematoxylin bluing solution (G1040, Servicebio), ultra-clean mounting medium (YZB, BASO). .. The following antibodies were obtained from the indicated suppliers: internal control primary antibody, mouse anti-β-actin (HC201, TransGen Biotech); target primary antibodies: rabbit anti-Csnk1g3-85aa synthesized by Hangzhou Huaan Biotechnology Co., Ltd, rabbit anti-CK1γ3 (AF9034, Affinity), rabbit anti-RIP1 (13176-AP, Proteintech), rabbit anti-RIP3 (AF7942, Affinity), rabbit anti-MLKL (OM155258, OmnimAbs), mouse anti-TNF-α (60291-1-lg, Proteintech), rabbit anti-IL-1β (sc-32294, Santa Cruz Biotechnology), rabbit anti-IL-6 (DF6087, Affinity); secondary antibodies: HRP-conjugated goat anti-rabbit IgG (H + L) (GB23303, Servicebio) and HRP-conjugated goat anti-mouse IgG (H + L) (GB23301, Servicebio).



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